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--- transfection [2022/12/19 04:32]
pamela [Chapter 1 - Genetic Engineering in Stem Cell Biomanufacturing]
+++ transfection [2022/12/19 08:34] (current)
liam
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-==== Graphine Based Gene Transfection ====
+==== Graphene Based Gene Transfection ====
 Abstract
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 Our results suggest graphene to be a [[:novel gene delivery nano-vector]] with low [[:cytotoxicity]] and high transfection efficiency, promising for future applications in non-viral based [[:gene therapy]]. ((https://web.archive.org/web/20220425062253/https://pubs.rsc.org/en/content/articlehtml/2011/nr/c0nr00680g?page=search))
+==== Jessica Rose Graphene Oxide Transfection ====
+Substack December 16,2022 by Jessica Rose
+{{ ::graphine_transfection_jessica_rose_graphic.png?400|}}
+Please refer to the published work in the journal ‘Nanoscale’ entitled: “Functionalized graphene oxide serves as a novel vaccine nano-adjuvant for robust stimulation of cellular immunity”.
+This work was published in 2016 by a group in China and let me tell you, they did a lot of work and asked a lot of questions. Their methods are so good, that I could actually reproduce their work.
+Their primary goal was to find out if their adjuvant antigen delivery system, graphene oxide-polyethylene glycol-polyethylenimine ([[:GO-PEG-PE]]I), would be functional with regard to specific immune stimulation, and/or less toxic than aluminum-based adjuvants.
+Their results showed that a PEG-PEI coated graphene oxide sheet - which incidentally really loves absorbing antigens like Ure B (an antigen associated with a bacterium called Helicobacter pylori) - was really good at trafficking antigen to dendritic cells to induce their maturation and activation when this concoction was injected intradermally.
+What I really need to get my hands on are the original methods… this isn’t even locatable using sci-hub. Anyone crafty enough to get this paper? I have the supplementary materials but not the text and will not be providing personal details to ‘sign up’ for anything to read science. It’s against my code
+Summary of paper translated into english: PEG-PEI-conjugated GO sheets used as adjuvants to deliver immune-stimulating agents like bacterial proteins, are a thing.
+((https://jessicar.substack.com/p/graphene-oxide-conjugated-to-peg))