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--- gain-of-function_research [2022/08/30 01:21]
pamela [Government Funding of GoF Research]
+++ gain-of-function_research [2022/09/16 16:16] (current)
pamela [peter daszak: supervillain origin story]
@@ Line 54: / Line 54: @@
 our story starts in 2014 when US bans were put in place on so called “gain of function” (GoF) research on viruses. such research seeks to deliberately modify viruses to make them more dangerous, either more deadly, more contagious, or both. contrary to what many seem to believe, this is not the same as a weapons program. ((https://boriquagato.substack.com/p/peter-daszak-supervillain-origin |Peter Daszak))
+==== Scientists struggles to recreate the SARS-CoV-2 furin cleavage site through infection ====
+July 20, 2022 {{ ::sars-cov-2_furin_cleavage_site_study_passage.png?600|}}
+The origin of the [[:furin cleavage site]] (FCS) in the SARS-CoV-2 S protein have long been a mystery. Now it seems to have became even more mysterious.
+Recently, a group of french Scientists attempted to passage one of the closest relatives of SARS-CoV-2 that can be cultured, BANAL-236, in models that are relative to human transmission and respiratory infection, what that was thought to be enabled by the furin cleavage site in the SARS-CoV-2 S protein, in the hope of trying to recreate the original emergence of the SARS-CoV-2 furin cleavage site that was thought to have triggered the current pandemic.
+This observation of the inability of SARS-like coronaviruses (Sarbecoviruses) to develop a functional furin cleavage site through the process of infection in-vivo or in-vitro, also raises a significant possibility that this site may have been the result of genetic engineering within the laboratory. In deed, even the group of french scientists themselves commented that “Alternatively, human infection might have occurred in the course of experimental virus isolation in the laboratory”.
+A restriction analysis of SARS-CoV-2 revealed that unlike it’s closest relatives, RaTG13 and the 5 BANAL-CoVs (BANAL-52, BANAL-103, BANAL-116, BANAL-236 and BANAL-247), two BsaI sites have been removed from the ORF1a, which were incidentally also located within, and therefore will interfere with, the F3 fragment of both of the two reverse genetic systems (RGS) of SARS-CoV-2 available in year 2020.
+During preliminary inoculation of cynomolgus macaques with BANAL-236, it was found that, unlike SARS-CoV-2, BANAL-236 infects primarily the intestines, and that shedding through the respiratory pathway was minimal, despite intranasal and intratraecheal inoculation being used in this inoculation test. It was therefore decided that a maximal of 6 serial passages in the CaCo-2 cell line and K18-hACE2 mice would be used to model the potential transmission route of BANAL-236 in humans, in order to simulate a worse-case scenario of human-to-human transmission through the intestinal and respiratory route, respectively. ((https://web.archive.org/web/20220802223943/http://adeno-news.com/2022/07/20/scientists-struggles-to-recreate-the-sars-cov-2-furin-cleavage-site-through-infection/))